Journal: Biomedicines
Article Title: The Role of Polymorphism in the Endothelial Homeostasis and Vitamin D Metabolism Genes in the Severity of Coronary Artery Disease
doi: 10.3390/biomedicines11092382
Figure Lengend Snippet: Serum level of endothelial homeostasis and vitamin D metabolism markers in the carriers of different polymorphic variants in the studied genes.
Article Snippet: Serum levels of E-, L- and P-selectins, endothelin, eNOS, 25OH vitamin D and 1.25-dihydroxy vitamin D were measured via enzyme-linked immunosorbent assay (ELISA) using Human sE-selectin Platinum ELISA Kit (eBioscience, San Diego, CA, USA), Human sL-selectin Platinum ELISA Kit (eBioscience), Human sP-selectin Platinum ELISA Kit (eBioscience), Endothelin-1 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA), Human eNOS DuoSet ELISA Kit (R&D Systems, USA), 25OH Vitamin D Total ELISA Kit (DiaSource Diagnostics, Louvain-la-Neuve, Belgium) and 1.25-Dihydroxy Vitamin D EIA Immunoassay (Immunodiagnostic Systems, East Boldon, UK) in accordance with the manufacturers’ protocols.
Techniques: